Researchers developed an innovative technique that integrates with vital cells for monitoring specific biomolecules for regulating healthy cell cultures for cell-based therapeutics.
Using microfluidic technology, the researchers were capable of coupling electrospray ionization mass spectrometry (ESI-MS) for virtual monitoring of precision quality control technique. The findings were published in the science journal Biotechnology and Bioengineering on September 10, 2018.
Andrei Fedorov, Woodruff Professor in the George W. Woodruff School of Mechanical Engineering at the Georgia Institute of Technology, said: “The way that the production of cell therapeutics is done today is very much an art. Process control must evolve very quickly to support the therapeutic applications that are emerging from bench science today. We think this technology will help us reach the goal of making these exciting cell-based therapies widely available.”
The new technique has potential to measure very low concentrations of specific compounds secreted by cells and could identify the biomolecules used to control cell health. The researchers plan to integrate their label-free monitoring directly into high-volume bioreactors, which will evidently help in producing a large number of cell that in turn will pave a way for providing for new therapies.
The researchers used advanced microfabrication techniques to create a microfluidic device, which assists in treating samples in less than a minute, while the convention techniques took hours to run the same processes. They monitored the small concentrations of large biomolecules by correlating the measurements of cell health and potency, which resulted in improving the manufacturing process of cell.